The sensitivity and specificity of the RSID-saliva kit for the detection of human salivary amylase in the Forensic Science Laboratory, Dublin, Ireland

David G Casey; Judy Price

doi:10.1016/j.forsciint.2009.10.009

The sensitivity and specificity of the RSID-saliva kit for the detection of human salivary amylase in the Forensic Science Laboratory, Dublin, Ireland

Forensic Sci Int. 2010 Jan 30;194(1-3):67-71. doi: 10.1016/j.forsciint.2009.10.009. Epub 2009 Nov 20.

Authors

David G Casey¹, Judy Price

Affiliation

¹ Forensic Science Laboratory, Garda Headquarters, Phoenix Park, Dublin 8, Ireland. dcasey@fsl.gov.ie

PMID: 19931992
DOI: 10.1016/j.forsciint.2009.10.009

Abstract

We demonstrate here that the RSID-saliva test can be used as a test for human salivary alpha-amylase on samples routinely examined in forensic casework. We show that the RSID-saliva test detects salivary alpha-amylase at lower concentrations than the Phadebas Quantitative test, that the RSID-saliva test does not cross-react with forensically important human fluids and that the RSID-saliva test can be successfully integrated into the whole swab semen extraction method.

Publication types

Validation Study

MeSH terms

Animals
Cats
Dogs
Feces / chemistry
Female
Forensic Medicine / instrumentation*
Humans
Male
Mice
Reagent Kits, Diagnostic*
Saliva / enzymology*
Semen / chemistry
Sensitivity and Specificity
Sheep
Species Specificity
Sweat / chemistry
Urine / chemistry
alpha-Amylases / isolation & purification*

Substances

Reagent Kits, Diagnostic
alpha-Amylases