[HSCI/HMS] Introduction to R and Differential Gene Expression Analysis workshop

Introduction to R & Differential Gene Expression Analysis workshop

(June 11^th - 13^th, 2018)

Description:

This 3-day hands-on workshop will introduce participants to the basics of R (using RStudio) and its application to differential gene expression analysis on RNA-seq count data. This workshop is intended to provide basic R programming knowledge. Participants should be interested in:

using R for increasing their efficiency for data analysis
visualizing data using R (ggplot2)
using R to perform statistical analysis on RNA-seq count data to obtain differentially expressed gene lists (DESeq2)

Workshop segments will address the following:

R syntax: Understanding the different 'parts of speech' in R; introducing variables and functions, demonstrating how functions work, and modifying arguments for specific use cases.
Data structures in R: Getting a handle on the classes of data structures and the types of data used by R.
Data inspection and wrangling: Reading in data from files. Using indices and various functions to subset, merge, and create datasets.
Visualizing data: Visualizing data using plotting functions in base R as well as from external packages such as ggplot2.
Exporting data and graphics: Generating new data tables and plots for use outside of the R environment.
Differential expression analysis for RNA-seq data:
- QC on count data
- Using DESeq2 to obtain a list of significant differentially expressed genes
- Visualizing expression patterns of differentially expressed genes
- Performing functional analysis on gene lists with R-based tools

In addition to the above, we will focus on good data management practices, installing & utilizing data packages from various sources, and the different ways to get help when coding in R.

** Please note that this workshop does NOT cover single-cell RNA-seq analysis. **

Prior experience with R or RNA-seq analysis is not required to attend this workshop.

Registration is now closed.

Please check the eligibility requirements below prior to registering!

Schedule:

9 AM - 5 PM on June 11^th, 12^th & 13^th(Monday through Wednesday).

There will be a short coffee break during the day and a longer lunch break (lunch will not be provided).

Cost and Registration:

There is a non-refundable $75 registration fee for this workshop.

We will be accepting 20-30 participants on a first-come, first-served basis:

If you are one of the first 20-30 eligible* registrants, you will receive an email with a link to pay the (non-refundable) registration fee.
If you are not among the first 20-30 eligible* registrants, you will be added to the waitlist and accordingly notified.

NOTE: You will not have a reserved seat for this workshop until you pay the registration fee. Please make sure you pay within the time stated in that email, else you will lose your spot to someone on the waitlist.

Registration is now closed.

Please check the eligibility requirements below prior to registering!

Eligibility Requirements:

*To be eligible to attend this workshop you should fulfill at least one of the following criteria:

Harvard Medical School researcher in a lab on the Quad, with grants administered by HMS OR
Harvard researchers whose PIs are associated with the HSCI as Principal or Affiliated faculty.

Questions?

Please contact us at hbctraining@hsph.harvard.edu with any questions.