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ISSN No:-2456-2165
Abstract:- Porphyromonas gingivalis is a major Antimicrobial photodynamic therapy (aPDT) has been
pathogen associated with initiation and progression of introduced as an alternative approach for antibacterial
periodontitis. therapy. It is a combination of 2 nontoxic ingredients, a
photosensitizer and light, which destruct the cell by
Aim and Objective photodamage, and finally leads to cell death. [3,4] A
The aim of the study is to compare effect of low- photosensitizer (a photoactivable material) attaches to the
level laser of wavelength 650nm on viability of target cells and gets stimulated by a proper wavelength of
porphyromonas gingivalis with and without light.
photosensitisers.
P. gingivalis , which has endogenous photosensitizer
Methods molecules such as porphyrins that in the presence of light
Thirty samples of bacterial suspensions (200µl) generate reactive oxygen species leading to bacterial killing.
were prepared and divided into six groups .Group 1-
laser group (low level laser with wavelength of 650nm, On the other hand, some studies using an exogenous
and irradiation time of 30 s), Group 2- Methylene blue + photosensitizer implicated the capacity of aPDT for
laser group (after pre-irradiation time of 10 min, laser killing P. gingivalis. [5] These photo sensitizers once
was irradiated), and Group 3- Toluidine blue O + laser stimulated by Low level laser (650 nm) releases free
group (after pre irradiation time of 10 min, LED was radicals of oxygen which are cytotoxic. [6].
irradiated), Group 4 -Methylene blue Group 5 -
Toluidine blue O (TBO) and Group 6- Control group This study was carried out to evaluate and compare the
(no treatment), Then, 20 μL of each sample was cultured influence of two different photosensitizing agents with Low
in blood agar plates and incubated for 72 hours in level laser on the viability of P. gingivalis .
microaerophilic atmosphere for colony counting.
II. MATERIALS AND METHODS
Result
The concentration of P. gingivalis was significantly The samples used for the study was ATCC- 33277
reduced after using the Methylene blue + laser and the strains of Porphyromonas gingivalis obtained from the
Toluidine blue O + laser group. (P values < 0.005) laboratory where the study was conducted. (Laboratory:
Central research laboratory, Maratha mandal dental college,
Conclusion Belgavi, Karnataka). The laser used was Baistra Portable
Within the constraints of this investigation, it can F3WW PAD Dental Low Level Laser model no
be deduced that photodynamic inactivation applying a 1600100100 (110v-220v )
laser and photosensitizers like Methylene blue and
toluidine blue was more efficient than photosensitizers P. gingivalis strains was suspended in thioglycolate
and laser irradiation alone in eliminating P. gingivalis. broth and bacterial density was visually adjusted to a
turbidity of 0.5 McFarland standard reagents. 200 µl of
Keywords:- Porphyromonas Gingivalis , Low Level Laser , bacterial suspension was transferred in to microtiter plates.
Methylene Blue , Toluidine Blue O. The wells of the microtiter plates were diluted with 1000 µl
distilled water. The wells were divided in to 6 groups.
I. INTRODUCTION
Group I: Laser alone (Wells contained 200 μL bacterial
Periodontal disease is an inflammatory process of the suspension and 200 μL broth. Then laser was irradiated
tissues surrounding the teeth. Bacterial plaque is the main to the wells.)
cause of periodontitis. [1] Porphyromonas gingivalis , a black
pigmented microorganism, is a major pathogen associated
with initiation and progression of periodontitis. [2]
Table 1 Shows Six Groups in Porphyromonas Gingivalis with Mean and Standard Deviation. P Value in
Comparison with Control Group
Porphyromonas gingivalis
n Mean + SD P value
GROUP 1 5 210.60 + 26.950 .000*
GROUP 2 5 .00 + .000 .000*
GROUP 3 5 .00 + .000 .000*
GROUP 4 5 342.00 + 35.249 .000*
GROUP 5 5 303.40 + 35.211 .000*
GROUP 6 5 432.20 + 12.853 control