The firefly luciferase reaction requires its substrate luciferin, plus adenosine triphosphate (ATP), O2, and Mg2+. The Renilla and Gaussia luciferases use ...

Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein.

Two types of luciferase genes, cloned from bacteria and firefly, are used as sensitive reporter systems in a wide variety of cells such as bacterial, yeast, ...

Aug 9, 2021 · Firefly luciferase (FLuc) and Renilla luciferase (RLuc) are two well-characterized luciferase reporter systems. FLuc was first cloned from the ...

Molecule of the Month: Luciferase. Organisms from fireflies to bacteria use luciferase to emit light. Firefly luciferase with the ...

Aug 9, 2021 · The split luciferase assay has been used to identify compounds which augment hepatitis B virus (HBV) assembly. (23) In the HBV life cycle, two ...

The system relies on two different reporter genes, Renilla and firefly luciferase, to evaluate regulated gene expression. The gene encoding Renilla luciferase ...

Jan 9, 2018 · A simple, accurate, sensitive and robust assay that can rapidly and specifically measure the death of target cells would have applications ...

Nov 15, 2021 · Bioluminescence imaging (BLI) using luciferase reporters is an indispensable method for the noninvasive visualization of cell populations ...

Mar 25, 2020 · A fungal bioluminescence pathway can be reconstituted in planta to create luminescence in many plant species without external substrate ...