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Sex education is often provided by public health experts that are contracted with schools to provide drop-in instruction. Using community-based participatory research (CBPR), we examined the effects of using universal design for learning (UDL) to better meet students’ needs. Thirty-one schools were randomly assigned to receive a standard or UDL versions of a four-day puberty lesson. Using a two-way multivariate analysis of covariance (MANCOVA), we identified students who received the UDL version had a greater sense of self-competence but no significant difference in knowledge when controlling for the school’s average knowledge at Time 1. There was not a differential effect for students in schools receiving Title 1 funding. Educators felt they had greater participation when using UDL but experience technological challenges.